pljm1 empty vector Search Results


plasmid vectors pljm1 empty  (Addgene inc)
94
Addgene inc plasmid vectors pljm1 empty
Plasmid Vectors Pljm1 Empty, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral constructs of pljm1-rragb q99l  (Addgene inc)
90
Addgene inc lentiviral constructs of pljm1-rragb q99l
Lentiviral Constructs Of Pljm1 Rragb Q99l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blast plasmid addgene rrid addgene 111887 recombinant dna reagent pljm1 empty  (Addgene inc)
98
Addgene inc blast plasmid addgene rrid addgene 111887 recombinant dna reagent pljm1 empty
Blast Plasmid Addgene Rrid Addgene 111887 Recombinant Dna Reagent Pljm1 Empty, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pljm1 empty vector  (Addgene inc)
92
Addgene inc pljm1 empty vector
Pljm1 Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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empty vector control hek293 cells  (Addgene inc)
96
Addgene inc empty vector control hek293 cells
a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed <t>HEK293</t> cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.
Empty Vector Control Hek293 Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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empty vector control hek293 cells - by Bioz Stars, 2026-02
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pbabe-haii  (Addgene inc)
90
Addgene inc pbabe-haii
a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed <t>HEK293</t> cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.
Pbabe Haii, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fugene hd  (Promega)
90
Promega fugene hd
a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed <t>HEK293</t> cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.
Fugene Hd, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reference mammalian selection pcdna3 1 1 empty vector control invitrogen  (Thermo Fisher)
86
Thermo Fisher reference mammalian selection pcdna3 1 1 empty vector control invitrogen
a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed <t>HEK293</t> cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.
Reference Mammalian Selection Pcdna3 1 1 Empty Vector Control Invitrogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
reference mammalian selection pcdna3 1 1 empty vector control invitrogen - by Bioz Stars, 2026-02
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pljm1 bsd blasticidin pmd2 g lentivirus generation addgene  (Addgene inc)
98
Addgene inc pljm1 bsd blasticidin pmd2 g lentivirus generation addgene
a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed <t>HEK293</t> cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.
Pljm1 Bsd Blasticidin Pmd2 G Lentivirus Generation Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lipofectamine 3000 transfection reagent  (Thermo Fisher)
86
Thermo Fisher lipofectamine 3000 transfection reagent
a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed <t>HEK293</t> cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.
Lipofectamine 3000 Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pljm1 flag ha ago2 wt  (Addgene inc)
93
Addgene inc pljm1 flag ha ago2 wt
a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed <t>HEK293</t> cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.
Pljm1 Flag Ha Ago2 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pljm1 mcs  (Addgene inc)
93
Addgene inc plasmid pljm1 mcs
a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed <t>HEK293</t> cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.
Plasmid Pljm1 Mcs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes

doi: 10.1038/s41467-023-41988-y

Figure Lengend Snippet: a YME1L, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). b Quantification of OPA1 Δ C protein level in ( a ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.000762, and 0.003106). c OMA1, OPA1 Δ C, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and OMA1 and OPA1 Δ C were incubated with and without the presence of FAM210A ( n = 3 independent experiments). d Quantification of OPA1 Δ C protein level in ( c ) (mean ± s.e.m; two-tailed unpaired Student’s t test). e Co-immunoprecipitation (Co-IP) of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments). f Proximity ligation assay (PLA) showing the protein interaction of FAM210A-FLAG and YME1L in Fam210a-flag overexpressed HEK293 cell line ( n = 3 independent experiments; scale bar: 10 µm). g YME1L, OMA1, and FAM210A were synthesized by E. coli cell-free protein synthesis system, and YME1L and OMA1 were incubated with or without the presence of FAM210A ( n = 3 independent experiments). h Quantification of OMA1 protein level in ( g ) (mean ± s.e.m; two-tailed unpaired Student’s t test; P = 0.02497). i Immunoblotting analysis of OPA1 in BAT of control and Fam210a iAKO after cold exposure for 0 h and 6 h by using the large format electrophoresis chamber ( n = 3 mice per group). j Quantification of OPA1-b/e in ( i ) (mean ± s.e.m; two-tailed paired Student’s t test; P = 0.02418). k Diagram depicting that FAM210A enhances YME1L activity thus suppressing OMA1, which protects L-OPA1 from over-cleavage during cold exposure to facilitate mitochondrial cristae remodeling (diagram created with BioRender.com). * P < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file.

Article Snippet: The Fam210a-flag overexpressed and empty vector control HEK293 cells were generated using pLJM-EGFP (Addgene #19319).

Techniques: Synthesized, Incubation, Two Tailed Test, Immunoprecipitation, Co-Immunoprecipitation Assay, Proximity Ligation Assay, Western Blot, Control, Electrophoresis, Activity Assay